Assessment of Kirsten Sarcoma Viral Gene Mutations in Non-neoplastic and Neoplastic Nodular Lesions of Thyroid in Pakistan
Farheen Jafri *
Department of Pathology, Jinnah Medical and Dental College, 22-23 Shaheed e Millat Road, Karachi, Pakistan
Shahnaz Imdad Kehar
Department of Pathology, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Center, Karachi, Pakistan
Kaniz Zehra Abbas
Office of Dr. Wajih Rizvi, New Jersey, USA
*Author to whom correspondence should be addressed.
Abstract
Objectives: To assess KRAS mutations in non-neoplastic and neoplastic nodular lesions of thyroid as the incidence of KRAS mutations in thyroid lesions in Pakistan has not been evaluated.
Methods: The cross sectional study was conducted at Basic Medical Sciences Institute, Jinnah Postgraduate Medical Center, Karachi from 2011 to 2014. 70 cases including 6 multinodular goiters, 10 hyperplastic nodules, 10 follicular adenomas, 7 WDT-UMP, 4 follicular carcinomas, 22 classical papillary carcinomas, 11 follicular variant of papillary carcinomas were subjected to standard PCR to detect KRAS mutations located at codon 12 exon 1.
Results: KRAS mutations located at codon 12 exon 1 were found in 02(2.87%) cases of multinodular goiter, 05(7.14%) hyperplastic nodules, 02 (2.87%) follicular adenoma, 03(4.2%) WDT-UMP. Among malignant lesions follicular carcinoma showed KRAS positivity 03(4.2%), classical papillary carcinoma 08(11.42%) and follicular variant of papillary carcinoma 07(10%).
Conclusion: Our data suggests strong presence of KRAS mutations in malignant tumors supporting the presence of KRAS mutations in our population particularly follicular variant of papillary carcinoma. Follicular variant is a discrete variant of papillary carcinoma having strong association with KRAS mutations.
Keywords: KRAS, FVPTC, RAS, WDT-UMP